P糖蛋白在胰腺癌中的表达及其与p21^(ras)和p53蛋白表达的关系

Expression of P glycoprotein in pancreatic cancers and its relationship with p21 ras and p53

为了解Ｐ糖蛋白（Ｐｇｐ）在原发和复发胰腺癌组织中的表达及其临床意义，并分析其表达与ｐ２１ｒａｓ和ｐ５３蛋白表达的关系。采用免疫组化法对４８例原发胰腺导管癌（其中１８例为胰腺癌淋巴结转移灶）和１５例复发胰腺癌的石蜡包埋标本进行Ｐｇｐ、ｐ２１ｒａｓ蛋白和ｐ５３蛋白单抗的免疫组化染色。其结果为４８例原发胰腺癌（其中１８例淋巴结转移灶）和１５例复发胰腺癌组织中呈Ｐｇｐ染色阳性分别为２１例（４３．８％），７例（３８．９％）和１１例（７３．３％），复发胰腺癌组织Ｐｇｐ阳性率高于原发癌（Ｐ＜０．０５）。Ｐｇｐ阴性组的原发癌患者经根治手术后平均生存期明显较阳性组长（Ｐ＜０．０１）。另外，原发癌Ｐｇｐ阳性组与ｐ２１ｒａｓ阳性组有相关性（Ｐ＜０．０５）而与ｐ５３阳性组则无相关性。结果提示Ｐｇｐ表达与胰腺癌对化疗的耐药性有关。

To investigate the expression of P glycoprotein (Pgp) in primary, metastatic and recurrent pancreatic adenocarcinomas; and to correlate P gp expression with p21 ras and p53 protein expression, histological samples from 48 primary pancreatic adenocarcinomas, 18 metastatic lymph nodes and 15 recurrent pancreatic cancers were studied immunohistochemically for the expression of Pgp, p21 ras and p53 proteins. Results showed membrane bound positivity of Pgp was observed in 21(43.8%) primary pancreatic adenocarcinomas, 7(38.9%) metastatic lymph nodes and 11(73.3%) recurrent cancers respectively. Expression of Pgp in recurrent pancreatic adenocarcinomas is higher than that in primary ones ( P <0.05). Among all the patients with primary pancreatic cancers, the mean survival time of negative group is obviously higher than that of the positive one. Besides, Pgp positive group was correlated with p21 ras positive group, while not correlated with p53 positive one. The results suggested (1)Pgp expression might be associated with clinical chemoresistence of pancreatic cancers. (2)Chemoresistence of p21 ras positive pancreatic cancers be probably mediated by Pgp expression.