胃癌细胞凋亡与p53蛋白异常相关性研究

Study on the correlation between apoptosis and over expression of p53 protein in gastric carcinoma

采用ＤＮＡ末端转移酶介导的Ｂｉｏ－ｄＵＴＰ原位末端标记技术（ＴＵＮＥＬ）和免疫组织化学技术，分别对６０例胃癌标本的细胞凋亡和ｐ５３蛋白异常作同步研究和相关性分析。结果在６０例胃癌组织中细胞凋亡指数为１．８％～１９．４％（平均９．３±３．９％），ｐ５３蛋白异常表达率为６３．３％（３８／６０）；ｐ５３蛋白阳性组细胞凋亡指数为３．７±１．１％（１．８％～７．９％），而ｐ５３蛋白阴性组细胞凋亡指数１４．７±２．４％（７．４％～１９．４％），两组间有显著性差异。结果表明胃癌中存在较高频率的ｐ５３蛋白异常和细胞凋亡。

We have examined the correlation between apoptosis and over expression of p53 protein in 60 specimens of human gastric carcinoma by the terminal deoxynucleotidyl transferaes mediated dUTP biotin nick end labeling (TUNEL) and immunohistochemical method. In the 60 specimens, apoptotic indices (number of apoptotic cells/total number of tumor cells) ranged from 1.8% to 19.4% (mean,9.3±3.9%), the rate of over expression of p53 protein was 63.3%(38/60). Moreover, apoptotic indices ranged from 1.8% to 7.9% (mean,3.7±1.1%) in p53 protein positive group and from 7.4% to 19.4% (mean,14.7±2.4%) in p53 protein negative group ( P <0.01). These results indicated the existence of over expression of p53 protein and high frequency of apoptosis in gastric carcinoma, which may both play an important role in the development of human gastric carcinoma.