PPARγ2基因Pro12Ala多态性与广东地区2型糖尿病的关系

Association between Pro12Ala polymorphism in peroxisome proliferator-activated receptor-γ2 gene and type 2 diabetes in Guangdong population

目的探讨广东地区人群中过氧化物酶增殖体激活受体-γ2(PPARγ2)基因Prol2Ala多态性与2型糖尿病的相关性。方法应用聚合酶链反应-DNA直接测序技术,对143例2型糖尿病患者和150例正常对照者PPARγ2基因Prol2Ala多态性进行基因分型。结果Prol2Ala多态性的基因型PP,PA和AA在2型糖尿病组和对照组的频率分别为93.0%、7.0%、0和90.7%、9.3%、0,P等位基因和A等位基因在2型糖尿病组和对照组中的频率分别为96.5%、95.3%和3.5%、4.7%,两组间的基因型和等位基因频率差异均无显著性(P>0.05)。2型糖尿病组中基因型为PA者体质指数(BMI)、空腹血糖(FBG)、总胆固醇(TC)、三酰甘油(TG)均高于PP基因型,但差异无统计学意义(P>0.05)。结论PPARγ2基因Pro12Ala多态性与广东地区2型糖尿病无明显关联。

Objective To investigate the correlation between Pro12Ala polymorphism in peroxisome proliferator - activated receptor - γ2 （ PPARγ2 ） gene and type 2 diabetes in Guangdong population. Methods Polymerase chain reaction （PCR） - direct sequencing assay was used to determine the distribution of allele and genotype frequencies of Pro12Ala polymorphism in PPARγ2 gene in 143 type 2 diabetic patients and 150 normal control subjects. Results The PP, PA and AA genotype frequencies of Pro12Ala polymorphism in 2 type diabetes and control group were 93.0%, 7.0% , 0% and 90. 7%, 9.3%, 0% respectively. Frequencies of P allele and A allele were 96. 5% vs 95.3% and 3.5% vs 4. 7% in type 2 diabetes and control groups respectively. There were no differences in genotype and allele frequencies between the two groups （ P 〉0. 05 ）. Ahhough the level of fasting blood sugar （FBG） , total cholesterol （TC） , triglyceride （TG） and body mass index （BMI） in 2 type diabetes with PA genotype was higher than those PP genotype patients, no significant difference was found. （P 〉 0.05 ）. Conclusion Pro12Ala polymorphism in PPARγ2 gene is not significantly associated with type 2 diabetes in Guangdong population.