肝癌术后检测血液AFP mRNA及AFP的临床意义

The clinical significance of combined examination of AFP and AFPmRNA in the blood after resection of hepatocellular carcinoma(HCC)

目的探讨检测患者血液甲种胎儿蛋白信使核糖核酸(alpha-fetoprotein messenger ribonucleic acid,AFP mRNA)及甲种胎儿蛋白(AFP)对判断肝癌术后复发或转移的临床意义。方法应用巢式 RT-PCR检测-4例肝癌患者术后2周、4周及6周时周围静脉血液中 AFP mRNA 及 AFP 的变化,并随访1年。结果 34例手术切除的肝癌患者中,术后6周时周围静脉血液中 AFP mRNA 仍呈阳性13例,术后6周内血液 AFP 持续阳性21例。术后1年内肿瘤复发或发现转移性病灶13例,其中7例血液 AFP mRNA及 AFP 均为阳性。结论术后检测血液 AFP mRNA 及 AFP 可更有效地判断肝癌的复发或转移。

Objective To study the clinical significance of blood AFPmRNA in prospecting postoperative recurrence and distant metastasis of human hepatocellular cacinoma (HCC).Methods We detected 34 blood samples from HCC patients by the time of 2 weeks,4 weeks and 6 weeks after operation by nested RT-PCR to find out AFPmRNA,and the patients were followed up for 1 year.Results AFPmRNA was detected in 13 blood sam- ples from 34 HCC cases and AFP were positive in 21 HCC patients out of 34 cases at 6 weeks after operation. Recurrence or distant metastasis were found within one year in 13 cases among these 34 patients (38.23%). Seven out of 13 cases with recurrent or metastatic carcinoma were both AFP mRNA and AFP positive at the time of 6 weeks postoperatively.Conclusions Combined postoperative AFPmRNA and AFP examination is more ef- ficient in predicting postoperative recurrence and metastasis.