知母中三个新的呋甾皂苷

Three New Furostanol Saponins from Anemarrhena asphodeliodes Bge.

从中药知母（ＡｎｅｍａｒｒｈｅｎａａｓｐｈｏｄｅｌｏｉｄｅｓＢｇｅ．）中分离出三种新的呋甾皂苷，初步鉴定为（２５Ｓ）－２６－Ｏ－β－Ｄ－葡萄糖－５β－呋甾－２０（２２）－双键－３β，２６－二醇－３－Ｏ－β－Ｄ－葡萄糖基（１→２）〔β－Ｄ－葡萄糖基（１→３）〕－β－Ｄ－葡萄糖基（１→４）－β－Ｄ－半乳糖苷（１），（２５Ｓ）－２６－Ｏ－β－Ｄ－葡萄糖基－２２－羟基－５β－呋甾－３β，２６－二醇－３－Ｏ－β－Ｄ－葡萄糖基（１→２）〔β－Ｄ－葡萄糖基（１→３）〕－β－Ｄ－葡萄糖基（１→４）－β－Ｄ－半乳糖苷（２），（２５Ｓ）－２６－Ｏ－β－Ｄ－葡萄糖基－２２－甲氧基－５β－呋甾－３β，２６－二醇－３－Ｏ－β－Ｄ－葡萄糖基（１→２）〔β－Ｄ－葡萄糖基（１→３）〕－β－Ｄ－葡萄糖基（１→４）－β－Ｄ－半乳糖苷（３）．分别命名为ｔｉｍｏｓａｐｏｎｉｎ－ＢⅣ，ｔｉｍｏ－ｓａｐｏｎｉｎ－ＢⅤ，ｔｉｍｏｓａｐｏｎｉｎ－ＢⅥ．

Three new furostanol saponins were isolated from Anemarrhena asphodeloides Bge.On the basis of chemical evidences and spectral analysis(1HNMR,13CNMR,DEPT),the structures of the 3 compounds were elucidated as (25S)26OβDglucopyranosyl5βfurost20(22)ene3β,26diol3OβDglucopyranosyl(1→2)〔βDglucopyranosyl(1→3)〕βDglucopyranosyl(1→4)βDgalactopyranoside(1),(25S)26OβDglucopyranosyl22hydroxy5βfurostane3β,26diol3OβDglucopyranosyl(1→2)〔βDglucopyranosyl(1→3)〕βDglucopyranosyl(1→4)βDgalactopyranoside(2),(25S)26OβDglucopyranosyl22methoxy5βfurostane3β,26diol3OβDglucopyranosyl(1→2)〔βDglucopyranosyl(1→3)〕βDglucopyranosyl(1→4)βDgalactopyranoside(3).They are all new compounds,named timosaponinBⅣ(1),timosaponinBⅤ(2),timosaponinBⅥ(3) respectively.