肾癌组织中细胞凋亡及相关基因bcl-2和bax表达的研究

Apoptosis of renal cell carcinoma and apoptotic related genes bcl 2 and bax

目的探讨肾癌的发病机制。方法采用原位ＤＮＡ片断末端标记和免疫组织化学方法，检测４８例肾癌组织细胞凋亡状态及凋亡相关基因ｂｃｌ２和ｂａｘ蛋白的表达。结果４８例肾癌组织中ｂｃｌ２蛋白低表达２２例，凋亡指数（ＡＩ）均值为１３５；高表达２６例，ＡＩ均值为１０４。两组ＡＩ均值间统计学分析差异有显著意义（Ｐ＜００５）。ｂａｘ蛋白低表达３４例，ＡＩ均值为１０３；高表达１４例，ＡＩ均值为１６３。两组ＡＩ均值间统计学分析差异有非常显著意义（Ｐ＜００１）。ｂｃｌ２蛋白表达越强，ｂａｘ蛋白表达越弱，凋亡细胞数就越少。结论ｂｃｌ２和ｂａｘ蛋白均参与了肾癌组织中细胞凋亡的调节。

Objective To explore the mechanism of renal cell carcinoma (RCC). Method We detected the apoptotic status and the expression of apoptotic related gene bcl 2 and bax protein in 48 cases of RCC with TdT mediated dUTP biotin nick end labeling (TUNEL) and immunohistochemistry methods. Result 22 cases showed low expression of bcl 2 protein with an average apoptotic index (AI) of 1 35 and 26 cases had high exprssion of bcl 2 with an AI of 1 04( P <0 05). 34 cases showed low expression of bax protein with average AI of 1 03 and 14 cases had high expression of bax protein with an average AI of 1 63 ( P <0 01). The stronger bcl 2 expression,the weaker bax expression and the less number of apoptotic cells. Conclusion both bcl 2 and bax take part in the occurrence and progerssion of RCC.