气相色谱法测定月见草籽超临界流体萃取物中有效成分的含量

Determination of Active Ingredient in the Supercritical Fluid Extractive Matter of Evening Primrose Seeds with Gas Chromatograph.

目的建立气相色谱-内标法测定月见草籽超临界流体萃取物中γ-亚麻酸含量的方法。方法采用高效毛细管柱气相色谱-内标法,FID检测器。结果γ-亚麻酸线性范围0.431～2.15μg/ml(R=0.9999,n=5),回收率98.75%(RSD=2.67%,n=6)。结论本方法方法简便、准确、稳定,可用于月见草籽超临界萃取物的质量控制。

Objective To establish a gas chromatograph（GC） method for the determination of γ - linolenic acid in the supercritical fluid extractive matter of evening primrose seed. Methods The γ - linolenic acid was determined by GC with capillary column and FID detector. Results The linear response range was 0. 431 -2. 15 μg/ml（R = 0. 9999,n = 5） , and the average recovery was 98.75% （RSD = 2.67% , n = 6）. Conclusion The method was rapid, accurate and stable for the quantifying control of the supercritical fluid extractive matter of evening primrose seeds.