哮喘患者尿液白三烯E_4的检验及临床意义

Clinical Significance of Measurement of Urinary Leukotrience E_4 (LTE_4) in Patients with Asthma.

目的探讨支气管哮喘患者尿液白三烯E4(LTE4)测定的临床意义。方法采用竞争性酶联免疫吸附实验技术检测28例哮喘患者急性发作期和非急性发作期的尿LTE4水平,并与健康对照组相比较;同时对哮喘患者的尿LTE4与第1秒用力呼气容积(FEV1)进行相关性分析。结果哮喘患者急性发作期尿LTE4明显高于非急性发作期及健康对照组(P<0.01),而非急性发作期又明显高于健康对照组(P<0.01)。急性发作期支气管哮喘患者尿LTE4和FEV1呈负相关(r=-0.534,p<0.01)。结论白三烯确实参与支气管哮喘的发生发展过程,通过检测支气管哮喘患者尿中LTE4水平,为支气管哮喘的诊断及治疗提供了有意义的参考指标。

Objective To evaluate the clinical significance of measurement of urinary leukotrience E4 （LTE4） in patients with asth- ma. Methods Urinary leukotriene E4 in 28 patients with asthma who experienced acute attack period and asymptomatic period and 18 controls was measured by ACETM competitive enzyme immunoassay and expressed as pg/mg creatinine. In addition, forced expiratory volume in one second was investigated in the patient in acute attack period. Results Urinary LTE4 in acute attack period was significantly higher than that in asymptomatic period （ P 〈 0.01 ） , and urinary LTE4 in asymptomatic period was significantly higher than that in the control group （P 〈 0. 01 ）. In acute attack period, there was a negative relation between urinary LTE4 and forced expiratory volume in one second （r = - 0. 534, P 〈 0. 01 ）. Conclusion Luekotrience is a useful maker of airway inflammation and can be helpful in guiding asthma management.