脑益嗪抗运动病时大鼠血浆PG和小脑Na^+K^+ATPase图像分析定量研究

Image and Quantity Analysis of Prostaglandin in Rats' Blood Plasma and Na + K + ATPase in Their Cerebellum During the Prevention of Motion Sickness by Cinnarizine

为探讨脑益嗪抗运动病作用机理，采用放射免疫方法和计算机图像分析系统，对运动病组（ＭＳＧ）和脑益嗪药物预防组（ＣＰＧ）大鼠血浆ＴＸＢ２、６ＫｅｔｏＰＧＦ１α和小脑毛细血管内皮细胞Ｎａ＋Ｋ＋ＡＴＰａｓｅ进行定量测量和分析研究。结果表明ＣＰＧ大鼠血浆ＴＸＢ２和６ＫｅｔｏＰＧＦ１α显著低于ＭＳＧ（ｐ＜００５），而小脑毛细血管内皮细胞Ｎａ＋Ｋ＋ＡＴＰａｓｅ活性则明显高于ＭＳＧ（ｐ＜００１）。作者认为，血浆ＴＸＢ２和６ＫｅｔｏＰＧＦ１α降低，与脑益嗪阻断血小板和血管内皮细胞Ｃａ２＋内流有关。脑内Ｎａ＋Ｋ＋ＡＴＰａｓｅ活性升高，可能是因为脑益嗪扩张脑血管、增加脑血流，阻滞Ｃａ２＋内流的结果。这些变化可视为脑益嗪抗运动病作用的重要机理。

To study the mechanism of cinnarizine in preventing motion sickness,TXB 2,6 Keto PGF 1α in rats' blood plasma and Na + K + ATPase activity in the endothelial cells of their cerebellar capillary were measured and analysed by a radioactive immunity analyser and a computer image system.The results showed that TXB 2 and 6 Keto PGF 1α in rats' blood plasma in the cinnarizine preventing group(CPG) decreased remarkably,compared with those in the motion sickness group(MSG) ( p<0.05) .The activity of Na + K + ATPase in the endothelial cells of rats' cerebellar capillary in CPG was higher than that in MSG ( p<0.01) .The authors suggest that the lower concentration of TXB 2 and 6 Keto PGF 1α in rats' blood plasma in CPG is closely related to cinnarizine which prevents Ca 2+ from entering into the platelets and into the endothelial cells of blood vessels.The higher activity of Na + K + ATPase in the cerebellum may be caused by cinnarizene which dilates the blood vessels in the brain,increases the blood flow therein,and hinders Ca 2+ from getting into the cerebellum cells.These change are believed to be the important mechanism of how cinnarizine prevents motion sickness.