氟化钠对5种常见龈下细菌抑制作用的体外研究

Research on Inhibition of Sodium Fluoride on Five Subgingival Bacteria in Vitro

采用试管二倍液体稀释法，测定氟化钠在体外厌氧环境对牙龈卟啉单胞菌、伴放线放线杆菌、中间型普氏菌、血链球菌和亲缘链球菌的最小抑菌浓度（ＭＩＣ），比较氟化钠对上述细菌的抗菌作用。结果显示：氟化钠对各实验细菌都有抑制作用，其中对牙周主要可疑致病菌牙龈卟啉单胞菌、伴放线放线杆菌、中间型普氏菌和龋相关菌亲缘链球菌有较明显的抑制作用，ＭＩＣ值为１２８～１０２４μｇ／ｍｌ，而对牙周有益菌血链球菌则需高浓度才有抑制作用，ＭＩＣ值为２０４８～４０９６μｇ／ｍｌ。

This in vitro study was aimed at observing inhibition action of fluoride on 5 common periodontal bacteria in subgingival microflora. The liquid media twofold dilution method was used to determine the minimal inhibition concentration (MIC) of sodium fluoride (NaF) to 9 strains of bacteria representing the main periodontopathic species(P. gingivalis, A. actinomycetemcomitans, P. intermedius) and the periodontal beneficial species (S. sanguis) and 1 strain of S. sorbrinus. The results showed that all the bacteria were inhibited at different concentration of NaF. The MIC values ranged from 128 μg/ml for P. gingivalis and A. actinomycetemcomitans, 256 μg/ml for S.sorbrinus, 1024 μg/ml for P. intermedius to 2048￣4096 μg/ml for S. sanguis. The results suggest that NaF can also be used as a periodontal antibacterium agent and those preparations containing about 1000 μg/ml fluoride can be used effectively against the periodontopathic bacteria without disturbing the local ecological balance.