基因重组乳链球菌防龋疫苗主动免疫防龋研究 Ⅲ.静脉内注射免疫孕兔的实验研究

A Study on Anticaries Activity of Genetic Recombinant Vaccine of Streptococcus lactis Ⅲ. Immunization in Vein with Recombinant S. lactis in Pregnant Rabbits

利用ＥＬＩＳＡ法观察携带有变形链球菌表面蛋白基因的重组乳链球菌ＨＬ１０７经静脉内注射免疫孕兔后诱导机体免疫应答情况。结果表明，免疫１周后，血清和乳清中特异性ＩｇＧ型抗体明显升高，并持续数周。提示重组乳链球菌ＨＬ１０７具有变形链球菌表面蛋白抗原ＰＡｃ的免疫原性。

The effects of immunization in vein with recombinant S.lactis HL107 carring the S.mutans surface protein PAc gene in pregnant rabbits were studied.The results indicated that specific antiPAc IgG in serum and milk were obviously induced 1 week after immunization and retained at high level for several weeks. It suggests that the recombinant S.lactis HL107 possessing immunogencity of S. mutans surface protein PAc is able to stimulate specific systemic immune response against PAc.