血液链球菌产生对氨基苯甲酸的实验研究 Ⅰ.反相高效液相色谱分析法的建立

Study on the Biosynthesis of Paraaminobenzoic Acid by S.sanguis Ⅰ. Establishment of a Reversedphase Highperformance Liquid Chromatography

建立一种测定对氨基苯甲酸的反相高效液相色谱分析法。结果：以间羟基苯甲酸（ＭＨＢＡ）为内标得到的最佳分离操作参数为：流率（Ｆ）＝１．５ｍｌ／ｍｉｎ，流动相＝磷酸盐缓冲液（ｐＨ＝２．２）∶改性甲醇＝９０∶１０，柱温（Ｔ）＝５５±２℃。该方法快速、灵敏、简便。

In order to study the synthesis of paraaminobenzoic acid (PABA) by S.mutans and the role of PABA in the interaction between S.mutans and S.sanguis, a reversedphase highperformance liquid chromatography (RPHPLC) for analysis of PABA was developed. The results showed that the optimal chromatographic parameters were flow rate of 1.5ml/min, T=55±2℃, methanol/phosphate buffer of 10/90(by vol) (pH 2.2 0.1mol/L), mhydroxybenzoic acid as an internal standard .The method is simple, rapid accurate and useful.