摘要
目的:探讨肝素和血小板源性生长因子(PDGF)对体外培养的人肾小球系膜细胞(MsC)胶原合成和分泌以及纤连蛋白(FN)、α1(IV)型胶原mRNA表达的影响。方法:应用3H-脯氨酸掺入、胶原酶消化法测定胶原含量;采用Northernblot法检测FN、α1(IV)型胶原mRNA。结果:对照组、肝素组及PDGF组细胞内胶原含量分别为(1.250±0.501)%,(0.809±0.161)%和(2.690±0.597)%,而其培养液中的含量分别为(1.608±0.512)%,(0.122±0.089)%和(3.870±0.647)%。上述结果显示肝素对该细胞合成和分泌胶原有抑制作用(P<0.05,P<0.01),而PDGF则具有明显促进作用(P均<0.01)。Northernblot分析结果显示肝素可抑制MsCFN、α1(IV)型胶原mRNA的表达,而PDGF则可明显增强其表达。结论:肝素对人肾小球MsC基质的合成和分泌有抑制作用,而PDGF则具有促进作用,其作用机制均发生在基因转录水平上。
Purpose To study the effect of heparin and plateletderived growth factor(PDGF) on synthesis and secretion of total collagen and expression of fibronectin (FN), α1 (IV) collagen mRNA in human cultured mesangial cells (MsC). Methods 3Hproline incorporation with collagenase digestion for mesurement of total collagen, and Northern blot analysis for detection of FN, α1 (Ⅳ) collagen mRNA. Results The total amount of collagen, newly formed and secreted by MsC were (1250±0501)%, (0809±0161)%, (2690±0597)% and (1608±0512)%, (0122±0089)%, (3870±0647)% in control, heparin and PDGF groups, respectively. Collagen values in heparin group were significantly decreased compared with normal group (P<005 or P<001), but increased in PDGF group (P<001). Northern blot analysis revealed that the expression of FN, α1(Ⅳ) collagen mRNA on MsC in heparin group were inhibited as compared to normal serum group, but enhanced in PDGF group. Conclusions The ECM synthesis and secretion are inhibited by heparin in human MsC, but stimulated by PDGF.
出处
《临床与实验病理学杂志》
CAS
CSCD
1998年第2期114-115,共2页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金
上海市教委重点学科基金
关键词
肾小球系膜细胞
肝素
PDGF
基质合成
分泌
glomerular mesangium
heparin
plateletderived growth factor
mesangial cell
collagen
fibronectins