卡维地洛抑制大鼠心脏成纤维细胞增殖及胶原合成的实验研究

Effect of Carvedilol on Proliferation and Collagen Synthesis of Cultured Cardiac Fibroblasts from Rats

【目的】探讨卡维地洛对的实验性大鼠（Wistar）心脏成纤维细胞（CFs）增殖及胶原合成的影响。【方法】以12周龄Wistar健康大鼠为研究对象。采用胰酶消化法培养CFs，采用改良的MTT比色法观察CFs的增殖状况，采用3H-胸腺嘧啶核苷（3H-TdR）掺入法测定CFs的DNA合成功能，3H-脯氨酸（3H-Proline）掺入法测定胶原合成。【结果]CFsMTT吸光值随卡维地洛浓度增加和作用时间延长而减低，并呈浓度和时间依赖性；0．01～10．00μmol／L卡维地洛分别干预72h时，Wistar大鼠CFs 3H-TdR及3H-Proline掺入率均较空白对照组明显减低（分别P〈0．05，P〈0．01）。随着卡维地洛干预浓度的增加，其抑制作用逐渐增强，呈浓度依赖性。【结论】卡维地洛可显著抑制CFs的DNA合成及细胞增殖，并进一步抑制胶原沉积及逆转左室肥厚。

[Objective] To observe the effect of carvedilol on proliferation and collagen synthesis in cardiac fibroblast （CFs） derived from Wistar rats. [Methods]Twelve-week old Wistar rats were used in this study. The CFs were isolated and cultured with the method of digestion method. DNA synthesis was measured by 3 H-TdR incorporation, and the cell number was determined by modified MTT array, and the collagen synthe- sis was measured by 3H-proline incorporation, [Results] MTT optical density（OD） value of CFs was decreased by carvedilol in a concentration-dependent and time-dependent manner. After 72-hour culture, with the increase of concentration of carvedilol on CFs, the 3 H-TdR and 3 H-proline incorporation from wistar rats were significantly decreased, compared with control group, （ P〈d0.05, P d0.01 ,respectively）, which showed con- centration-dependent effects. [Conclusion]Carvedilol can inhibit significantly DNA synthesis and proliferation, and then inhibit collagen synthesis and attenuate left ventricular hypertrophy of hypertension.