蚯蚓纤溶酶对犬纤溶酶活性和血小板聚集功能的影响

EFFECT OF EARTHWORM FIBRINOLYTIC ENZYME GIVEN ORALLY ON THE ACTIVITY OF PLASMIN AND ON PLATELET AGGREGATION IN BEAGLES DOGS

目的研究口服蚯蚓纤溶酶（ＥＦＥ）对纤溶系统和血小板聚集功能的影响。方法正常雄性Ｂｅａｇｌｅ犬，体重６．５～９ｋｇ，经口给蚯蚓纤溶酶，经前肢静脉取血。以Ｓ－２２５１为发色底物，测定血浆纤溶酶活性；５０μｍｏｌ／ＬＡＤＰ为诱导剂测定血小板聚集率。结果（１）单剂量ＥＦＥ４０、８０ｍｇ／ｋｇ后血浆纤溶酶活性升高（Ｐ＜０．０５），８０ｍｇ／ｋｇ组高于４０ｍｇ／ｋｇ组（Ｐ＜０．０５）。连续给药１０ｄ，４０、８０ｍｇ／ｋｇ组均有多个时间点纤溶酶活性升高（Ｐ＜０．０５），但两组间无差异。（２）单剂量ＥＦＥ４０ｍｇ／ｋｇ，或经口给阿司匹林８ｍｇ／ｋｇ后３ｈ，血小板聚集均被抑制（Ｐ＜０．０５）；而在单剂量ＥＦＥ８０ｍｇ／ｋｇ或经口给淀粉后３ｈ，血小板聚集功能均无变化。结论ＥＦＥ经口给药可提高血浆纤溶酶活性，并在一定剂量下可抑制血小板的聚集。

URPOSE To study the effect of orallyadministered earthworm fibrinolytic enzyme (EFE) on the activity of plasmin and platelet aggregation.METHODS The healthy Beagles dogs (6.5～9kg) were orally administered with a single/multiple 40 or 80mg/kg dose of EFE, and venous blood was drawn for determination. The activity of plasmin was determined by means of chromogenic substrate S-2251. ADP 50μmol/L was used as an inducer to measure the platelet aggregation.RESULTS (1) After the first dose of EFE administration, the activity of plasmin of Beagles dogs was increased in a dosedependent manner (P<0.05). Similar increase was observed with successive oral administration for 10d, but there was no significant difference between the 40mg/kg and 80mg/kg groups. (2) The aggregation of platelet was inhibited by EFE 40mg/kg and aspirin 8mg/kg 3h after administration (P<0.05). No significant effect was found while EFE 80mg/kg and amylum was administered orally.CONCLUSIONS EFE increases the activity of plasmin and inhibits platelet aggregation in normal Beagles dogs after oral administration.