抗纤益心方对扩张型心肌病大鼠心肌细胞凋亡及Bax、Bcl-2蛋白表达的影响

Effect of Kangxianyixin prescription on cardiocyte apoptosis and expression of Bax and Bcl-2 in rRats with dilated cardiomyopathy

目的:观察抗纤益心方对扩张型心肌病大鼠心肌细胞凋亡及Bax、Bcl-2蛋白表达的影响。方法:喂饲呋喃唑酮建立扩张型心肌病动物模型,随机分组后药物干预8周,检测心肌凋亡及心肌Bcl-2、Bax蛋白表达。结果:①中西药联用组、抗纤益心方大剂量组、卡托普利组、抗纤益心方小剂量组心肌细胞凋亡指数与模型组比较有显著差异(P<0.05),其中以中西药联用组减少凋亡最明显;②与模型组相比,中西药联用组、大剂量组和卡托普利组均能明显下调Bax蛋白表达(P<0.05),上调Bcl-2蛋白表达(P<0.05)。结论:抗纤益心方有抑制或减少扩张型心肌病大鼠心肌细胞凋亡的作用,其机制与下调Bax蛋白表达、上调Bcl-2蛋白表达有关。

Objective：To expound the effect of Kangxianyixin prescription on cardiocyte apoptosis and expression of Bax and Bcl-2 in rats with dilated cardiomyopathy. Methods： Wistar rats with dilated cardiomyopathy established by furazolidone were treated 8 weeks with Kangxianyixin prescription, to observe cardiocyte apoptosis and to measure Bax and Bcl-2 expression in rats, Results： （1） In comparison of cardiomyocyte apoptosis indexes in rats, when compared with model group, the results showed significant difference in the combined group of high dosage of Kangxianyixin prescription and captopril, the high dosage group, the low dosage group, the captopril group （P〈0.05） ; （2） Compared with the model group, the combined group of high dosage of Kangxianyixiu prescription and captopril, the high dosage group and the captopril group could dramatically lower Bax gene expression （P〈0.05）and increase Bcl-2 gene expression（P〈0.05）. Conclusion： Kangxianyixin prescription can restrain or decrease cardiomyocyte apoptosis, it may be interrelated with reducing Bax expression and increasing Bcl-2 expression.