中药骨康对去势大鼠骨质疏松作用机制的实验研究

Experimental study on effect of GuKang on osteoporosis rats induced by ovariectomy

目的:探讨中药骨康对雌性大鼠切除卵巢后所致骨质疏松的作用机制。方法:6月龄的雌性SD大鼠随机分为手术模型组、雌激素组、中药组和空白对照组。造模成功后,中药组给予补肾中药骨康灌胃;雌激素组给予尼尔雌醇灌胃;手术模型组和空白对照组给予蒸馏水灌胃。治疗3个月后,分别测量各组大鼠的体质量、骨密度和外周血清中Ⅰ型前胶原羧基末端前肽(PICP)、Ⅰ型胶原交联羧基末端肽(ICTP)和雌激素(E2)的含量。结果:治疗后中药组、雌激素组的活体全身骨密度、腰椎骨密度增加,与手术模型组比较,有明显的统计学意义(P<0.05);中药组与雌激素组能显著降低ICTP(P<0.05),提高PICP和E2的含量(P<0.05)。结论:中药骨康对卵巢切除所致的骨质疏松症有明显的治疗作用,其机制可能是在一定程度地提高了体内的雌激素水平,抑制骨的吸收,促进骨的形成。

Objective： To study the mechanism of GuKang on osteoporosis rats induced by ovariectomy. Methods： 48 six-month-old female rats were divided into 4 groups： normal control group, ovariectomized group, estrogen group and traditional chinese herbs group. After establishing model, the rats of traditional chinese herbs group were given the intragastric administration with GuKang. The rats of estrogen group were given Nilestriol, other groups were given distilled water. After treatment for 3 months, the bone mineral density （BMD）, the body weight, the content of carboxyterminal propeptide of type I procollagen （PICP）, carboxyterminal telopeptide of type I collagen（ICTP） and E2 were measured. Results： Compared with ovariectomized group, the BMD of all over the body and lumbar vertebrae in both traditional chinese herbs group and estrogen group increased obviously （P〈0.05）. Moreover, compared with the ovariectomized group, traditional chinese herbs can reduce the volume of ICTP, and increase the content of PICP and E2 inversely （P〈0.05）. Conclusion： Gukang had the obvious treatment function to ovariectomyinduced osteoporosis, its mechanism was to a certain extent improving the body＇s estrogen level, which inhibited bone resorption and promoted bone formation.