脑泰方提取物对局灶性脑缺血大鼠血管内皮生长因子表达的影响

Effects of Naotaifang extracts on expression of VEGF after focal cerebral ischemia in rats

目的:探讨脑泰方提取物对大鼠局灶性脑缺血后血管内皮生长因子(VEGF)及VEGF mRNA的影响。方法:30只SD大鼠随机分为假手术组、模型组、脑复康组、脑泰方低、中、高剂量组。通过月桂酸钠损伤血管内皮细胞来复制局灶性脑缺血模型,采用免疫组化和RT-PCR法检测各组大鼠脑内VEGF阳性细胞数和VEGF mRNA的表达,同时评价大鼠神经功能。结果:脑缺血后,药物组神经功能症状得到改善,尤以脑泰方中剂量组变化最显著(P<0.01);假手术组大鼠脑内有少量VEGF阳性细胞,脑缺血后VEGF阳性细胞表达增加,与模型组比较,脑泰方中剂量组VEGF阳性细胞数明显增多(P<0.01),而且VEGF mRNA的表达也显著加强(P<0.01)。结论:脑泰方提取物增强大鼠局灶性脑缺血后VEGF及VEGF mRNA的表达,可能是其抗脑缺血的作用机制之一。

Objective： To approach the effect of Naotangfang extracts on expression of vascular endothelial growth factor （VEGF） after focal cerebral infarct in rats. Methods： 30 Sprague-Dawley （SD） rats were randomly divided into 6 groups： sham operation group, model group, Naotaifang extract groups （low, middle, and high dosage）, and Naofukang group. Some indexes were detected such as neurological scores, VEGF rnRNA expression by RT-PCR, and the number of VEGF positive cells by immunohistochemical methods. Results： The symptoms in nervous function were improved in the Admin. groups, especially in the Naotaifang middle dosage group. The number of VEGF positive cells and VEGF PCR production in Naotaifng middle dosage group was higher than that in the model group. Conclusion： Naotaifang has neuroprotective effect against focal cerebral ischemic injury by improving the expression of VEGF.