微波辅助提取麦冬黄酮的工艺研究

Study on microwave-assisted extraction technique of flavonoid from Ophiopogon japonicus

目的:研究微波辅助法提取麦冬黄酮的工艺条件。方法:采用单因素试验,探讨料液比、微波处理时间、微波强度以及乙醇体积分数对麦冬黄酮提取量的影响,并与传统回流法进行比较。结果:微波提取优化工艺条件为:乙醇体积分数为90%,料液比为1:14(g/ml),微波功率为中档,微波提取时间4min。微波处理后再进行回流提取较不经微波处理得到提取物的黄酮含量高出39.7%。结论:微波辅助法是一种提取麦冬黄酮的有效方法。

Objective： To study microwave-assisted extraction technique of flavonoids from Ophiopogon japonicus. Methods： The effect of the parameters, i. e. ratio of solid to liquid ,microwave treatment time, microwave power and volume ratio of ethanol on extraction amount of flavonoid were analyzed by single factor experiment. Results： Optimum processing data were determined as follows： 90.0% ethanol as extracting solvent, stock ratio 1：14（g/ml）, microwa＇~e extracting for 4 minutes at the power of MED. The extraction rate was 39.7 % higher than that of non-microwave＇ s.Conclusion： Extraction of flavonoids by microwave from Ophiopogon japonicus is an eficient method.