人大颗粒淋巴细胞抗生素肽的部分纯化

Partially Purified Antibacterial Polypeptides from Granules of Human Large Granular Lymphocytes

为探讨人大颗粒淋巴细胞抗菌分子的抗菌活性，采用制备性酸性尿素聚丙烯酰胺凝胶电泳对人大颗粒淋巴细胞胞浆颗粒酸溶性提取物的抗菌组分ＨＬＰ１、ＨＬＰ２、ＨＬＰ３进行分离纯化。结果：ＴｒｉｃｉｎｅＳＤＳＰＡＧＥ分析显示，ＨＬＰ１、ＨＬＰ２均为多肽混合物，其分子量范围分别是５６～１３ｋｄ和５６～８８ｋｄ；ＨＬＰ３为一７ｋｄ的单一多肽。琼脂糖弥散法和电泳凝胶琼脂糖弥散法抗菌试验显示，ＨＬＰ１、ＨＬＰ２、ＨＬＰ３对致病性大肠杆菌ＭＬ３５Ｐ氨苄青霉素耐药株、金葡球菌ＡＴＣＣ２５９２３株、绿脓杆菌ＡＴＣＣ２７８５３株均有很强的杀菌作用。

This was a study on the antibacterial activity of human large granular lymphocytes (LGL). In our previous work, three antibacterial fractions named HLP 1,HLP 2 and HLP 3 had been identified from the acid soluble extracts of the granules of LGL by using the gel overlay technique. The present study demonstrated that HLP 1, HLP 2 and HLP 3 separated by preparative acid urea polyacrylamide gel electrophoresis were potently bactericidal against E.coli MI 35P, S. aureus ATCC 25923, P.aeruginosa ATCC 27853 when tested for their antibacteiral activities by using both agarose radial diffusion assay and gel overlay technique. SDS PAGE analysis showed that the HLP 3 was almost purified with the molecular weight of 7kd. The HLP 1 and HLP 2 were more complex, and the ranges of molecular weight were 5.6 13kd and 5.6 8.8kd respectively. Our study suggests that the granules of human LGL might contain some low molecular weight antibiotic peptides which play an important role in human innate immunity.