清胰化积方联合IL-2对胰腺癌小鼠脾淋巴细胞功能的影响

Effect of Qingyi Huaji formula combined with IL-2 on immune function of splenic lymphocytes in mice with pancreatic cancer

目的:研究清胰化积方联合白细胞介素-2(IL-2)对Panc02胰腺癌小鼠脾淋巴细胞功能的影响。方法:荷瘤小鼠随机分为对照组、清胰化积方组、清胰化积方联合不同剂量的IL-2组,治疗后称量瘤质量并计算抑瘤率,分别以淋巴细胞增殖活性和转化率评价小鼠脾淋巴细胞功能。结果:中药组、中药+IL-2小、中、大剂量组的瘤质量均小于对照组(P<0.01),抑瘤率分别为37.84%、44.14%、38.74%和39.64%;各治疗组脾淋巴细胞增殖活性和转化率均高于对照组(P<0.01),并呈现IL-2的剂量依赖性,其中中药+IL-2大剂量组最高,与中药组比较有显著性差异(P<0.05)。结论:增强脾淋巴细胞免疫功能是清胰化积方联合IL-2抗小鼠胰腺癌的机制之一;清胰化积方联合IL-2的免疫效应优于单用清胰化积方,具有协同效应。

Objective： To study the effect of Qingyi Huaji （QYHJ） formula combined with interleukin-2（IL-2） on immune function of splenic lymphocytes in mice bearing pancreatic cancer Panc02. Methods： Tumor-bearing mice were divided into control group, QYHJ group and groups of QYHJ combined with IL-2 at different dosages at random. After treatment, tumor weights were measured and tumor-inhibition rates were calculated, immune function of mice＇s splenic lymphocytes were evaluated by proliferation activity and transformation rates. Results： The tumors weight in QYHJ group and groups of QYHJ plus IL-2 at low, medium and high dosages were lighter than that in control group （P〈0.01）, the tumor-inhibition rates were 37.84%, 44.14%, 38.74% and 39.64% respectively. The proliferation activity and transformation rates of splenic lymphocytes in all treatment groups were higher than those in control group（P〈0.01）, they showed dose dependent effect of IL-2, the differences between QYHJ group and group of QYHJ plus high dose IL-2 were significant （P〈0.05）. Conclusion： QYHJ formula combined with IL-2 may inhibit the growth of mice pancreatic cancer, one of the mechanisms is enhancing the immune function of splenic lymphocytes; the immunological effect of QYHJ formula combined with IL-2 is better than single QYHJ Formula, they may have synergism.