口腔修复材料表面粗糙度与血链球菌早期粘附的关系

Effects of Surface Roughness of Two Restorative Materials on Early Streptococcus Sanguis Adhesion in Vitro *

口腔修复材料表面粗糙度影响微生物粘附的问题，因长期缺乏令人信服的客观实验依据，一直未能受到应有的重视。此研究采用表面轮廓仪量化测定了口腔义齿修复材料（湿热固化基托塑胶及ＣｏＣｒ合金）的表面粗糙度，通过将不同粗糙度级的试件与口腔血链球菌在体外厌氧孵育２４小时后，测定细菌粘附量，并用扫描电镜观察试件表面形态及细菌粘附情况。结果显示血链球菌的２４小时粘附量与修复材料表面粗糙度呈正相关关系，相关系数分别为ｒ塑胶＝０４６（Ｐ＜００１），ｒ合金＝０２５（Ｐ＜００１）。从而提示临床修复体的打磨抛光应尽可能获得光滑表面，以期能在最大程度上减小其表面的菌斑积聚量。

This was an in vitro study aimed at the surface roughness of two kinds of commonly used restorative materials resin and alloy necessary to affect the adhesive behavior of Streptococcus sanguis(S.s) on them after 24h. The surface roughness(SR) of each tested sample was evaluated with a profilometer quantitatively and observed with scanning electron microscope (SEM) morphologically. Then the adhesive microbial amount was determined by the clone forming unit counting method, and adhesion morphology was analyzed with SEM. The result showed a positive linear relation between the adhesion amount of S.s and the SR. The relative coefficients were r resin =0.46( P <0 01) and r alloy =0.25( P <0 01) respectively. These suggest the SR of the restorative material is important for the early adhesion of oral microbes in vitro. Therefore, before the prothesis is inserted in the patient's oral cavity, its surface should be polished as smoothly as possible so that the bacterial adhesive amount on its surface can be decreased, and hence the patient may keep in good oral health and have a prolonged use of the prothesis.