盐酸氟桂嗪及维生素C对离体大鼠心脏缺血再灌注损伤的保护作用

Protective Effects of Flunarizine and Vitamin C on Isolated Rat Heart Subjected to Ischemia Reperfusion Injury

采用Ｌａｎｇｅｎｄｏｒｆ离体大鼠心脏灌注，研究全心缺血１０分钟、再灌注１５分钟后心肌细胞内Ｃａ２＋、丙二醛（ＭＤＡ）含量，冠脉流出液中乳酸脱氢酶（ＬＤＨ）含量以及心肌形态学改变。进而观察盐酸氟桂嗪（ＦＮＺ）和／或维生素Ｃ（ＶｉｔＣ）对上述各指标的影响。结果显示：再灌注１５分钟时ＦＮＺ＋ＶｉｔＣ组、ＶｉｔＣ组和ＦＮＺ组的心肌细胞内Ｃａ２＋含量无显著性差异（Ｐ＜００５），但心肌细胞内ＭＤＡ含量以及冠脉流出液中ＬＤＨ含量均比对照组低（Ｐ＜００５和＜００１），形态学改变也比对照组轻，ＦＮＺ和ＶｉｔＣ联用时效果更显著。由此表明，ＦＮＺ和ＶｉｔＣ均可通过抑制细胞内钙超载和氧自由基损伤而减轻再灌注损伤。

Isolated rat heart was subjected to Langendorff perfusion for 10 minutes of total global ischemia, and reperfusion for 15 minutes. The content of myocardial intracellular calcium, content of oxygen free radical induced lipid peroxide mitabolite malondialdehyde (MDA), content of Lactic dehydrogenase (LDH) in coronary effluent, and the change of myocardial morphology were studied. The effects of flunarizine(FNZ) and/or vitamin C(Vit C) on the above parameters were observed. The results showed that, at 15 minutes of reperfusion, the contents of myocardial intracellular calcium, of MDA in myocaredium and of LDH in coronary effluent in the FNZ+Vit C group, Vit C group and FNZ group were all lower than those in the control group, and the change of morphology in the three groups was also slighter than that in the control group. When FNZ and Vit C were administered together, the effect was more marked and equivalent to the sum total of the effects of the two drugs. These suggest that both FNZ and Vit C reduce reperfusion injury through suppression of myocardium calcium overload and oxygen free radical damage.