应用花粉管通道技术选育超级稻龙粳14试验研究

Study on Application of Pollen-Tube Pathway Transformation to Breeding Longjing 14, a Super Rice Variety

应用花粉管通道技术,以玉米黑301总DNA溶液为供体导入寒地水稻28个组合,结果表明:D1代表现与受体表型相同,D2代仅在转化龙粳4号的组合中出现3个变异植株;从其遗传变异规律看,变异植株可能由玉米黑301总DNA转化引起,但目前还缺乏直接的分子证据;经过连续定向选择,现已育成超级稻新品种龙粳14号审定推广;组合的遗传转化率看似很高,但从颖花数的遗传转化率看确很低,尤其是花粉管通道技术应用的很多物质和环境条件的不确定性,导致了试验存在很大的偶然性,试验的重演性差。

Using pollen-tube pathway transformation technique, the genomic DNA of Hei 301, a maize cultivar, as donor was introduced into 28 rice combinations. The results showed that the phenotype of D1 generation was the same as receptor and D2 generation only found three mutant plants in transformed Longjing 4. According to inheritance principle, the mutation resulted from transformation of genomic DNA of Hei 301, but it is lack of molecular identification. However, through continuous selection, the rice variety Longjing 14 was bred and registered. Although the transformation rate based on comb/nation seemed highly, the transformation rate of spikelets was very low. Because of uncertain material and environment in pollen-tube pathway transformation method, the contingency occurred in the experiment and led to poor replication.