丹参注射液对豚鼠耳蜗缺血再灌注损伤后细胞凋亡的影响

Effect of Danshen Injection on Apoptosis in Cochlear of Guinea Pigs After Ischemia Reperfusion

目的研究丹参对豚鼠耳蜗缺血再灌注损伤后细胞凋亡的影响。方法将72只健康豚鼠随机分成正常组、假手术组、丹参干预假手术组、模型组和干预组,采用组织化学法观察缺血再灌注不同时间点耳蜗各部位的组织学改变;用原位凋亡法观察耳蜗缺血及不同时间段内再灌注的细胞凋亡情况。结果正常组、假手术组、丹参干预假手术组豚鼠内耳罕见凋亡细胞,模型组Corti器、血管纹、螺旋神经节在缺血及再灌注各个时间点上均有细胞凋亡;干预组Corti器、血管纹、螺旋神经节在缺血及再灌注各个时间点上均有细胞凋亡,凋亡指数较模型组降低(P<0.05)。结论丹参使凋亡细胞减少,对缺血再灌注损伤后的耳蜗起保护作用。

Objective To investigate the effect of Danshen injection on the apoptosis in cochlear of guinea pigs after ischemia repeffusion. Methods 72 healthy guinea pigs were randomly divided into the normal group, sham operation group, sham operation group after the application of Danshen injection, model group and drug treated group, the last group was given intramuscular injection with Danshen injection 8 ml/kg before operation for 7 days, the samples of the last 2 groups were taken at 30 rain, 6 hours and 24 hours after repeffusion respectively. The histological changes in each parts of cochlear at different time points were ob- served by histochemistry and the apoptosis was studied by TUNEL. Results There was little apoptosis in the normal group, sham operation group and sham operation group after the application of Danshen injection, but there were more apoptosis in the corti organ, stria vaseularis, ganglion spirale of cochlear at each time point after ischemia or repeffusion in model group and drug treated group. The apoptosis index in model group were lower than that in drug treated group （P〈0.05）. Conclusion Danshen can inhibit apoptosis and protect the cochlear after ischemia repeffusion.