摘要
在斑节对虾Penaeus monodon催熟催产过程中,常用的方法是切除眼柄。此实验选用体重为149.5±25.8g的自然海区斑节对虾,比较了注射血清素(serotonin)和切除眼柄2种处理方法对斑节对虾的催熟效果。血清素注射剂量为50μg.g-1体重,在实验的第1、5和10天注射,共计3次。结果显示,2种方法处理后,受试亲虾性腺指数(gonad somatic index,GSI)显著高于对照组,同时注射血清素的实验组亲虾的存活率及其卵子孵化率显著高于切除眼柄的实验组。实验结果表明,血清素对斑节对虾催熟有促进作用,但发挥效应所需要的时间较长;从切除眼柄手术到第一次产卵所需时间较短,但是手术后的亲虾死亡率较高。
Eyestalk-ablation is used usually to induce ovarian maturation in Penaeus monodon. Wild P. monodon females were obtained from the sea around Sanya, weighted 149. 5 ± 25.8 g. Serotonin solution was injected into broodstoek at 50 μ·g^-1 body weight on the 1 ^st, 5^th, 10^st day. Survival and hatching rate were significantly higher in serotonin-injected females than unilateral eyestalk-ablated shrimp. But the time to the first spawning event from the beginning of treatment was shorter for eyestalk-ablated females. Gonad somatic index (GSI) in serotonin-injeeted and eyestalk-ablated groups were both significantly higher than the control. The results suggested that stimulation of serotonin can enhance the ovarian maturation of P. monodon.
出处
《南方水产》
2009年第1期59-63,共5页
South China Fisheries Science
基金
国家高技术研究发展计划(863计划)项目(2006AA10A406)
关键词
血清素
切除眼柄
班节对虾
催熟
serotonin
eyestalk-ablation
Penaeus monodon
maturation