摘要
为了研究皖西白鹅生殖系统内是否有产生Ghrelin细胞分布,应用免疫组化SABC法并结合DAB显色技术,结果:①在卵巢的生长卵泡中,均有ghrelin免疫阳性细胞,尤其在生长卵泡细胞的颗粒层更为明显;闭锁卵泡的卵泡外腺细胞也呈现ghrelin免疫阳性反应;②在输卵管五段的粘膜层中均观察到ghrelin免疫阳性细胞,其中,漏斗部细胞着色最深,数量最多,膨大部细胞反应最微弱,峡部、子宫部和阴道部ghrelin免疫反应介于二者之间,肌层和外膜均未见ghrelin表达。结论:产生Ghrelin的细胞在成年皖西白鹅卵巢和输卵管中均有广泛的分布,揭示Ghrelin可能调节生殖功能。
To study the distribution of ghrelin-producing cells in reproductive system of WanXi white geese. Methods：The immunohistochemical SABC method and DAB staining technology were adopted. Results： Ghrelin immunopositive cells （ghrelin-ip） were mainly found in the granularl layer of the grow follicles. Atretic follicle showed specific ghrelin immunostaining, especially follicular grand cells. Ghrelin-ip cells were found in the mucous membrane of tuba uterine. Strong and more ghrelin-ip cells were observed in the infundibulum part , weak ghrelin staining cells in the magnum part, but moderate ghrelin staining cells in isthmus part, utera part and vigina part . No immunoreactive cells were found in the tunica muscularis and tunica adventitia. Conclusion：The results suggested that ghrelin-producing cells exist in the ovary and tuba uterine of the adult WanXi white goose, which indicates that ghrelin may regulate the function of the reproduction.
基金
安徽省省自然科学基金(070411015)
