摘要
目的探讨大肠癌组织中基质金属蛋白酶-9（MMP-9）及其抑制物基质金属蛋白酶抑制剂-1（TIMP-1）的表达和临床意义。方法采用PowerVision二步法免疫组织化学染色检测42例大肠癌组织及其癌旁组织中MMP-9和TIMP-1表达情况并分析其与临床病理参数的关系。结果MMP-9在大肠癌组织中表达率明显高于癌旁组织（69．1％与45．2％，P=0．009）；TIMP-1在大肠癌组织中表达率明显高于癌旁组织（61．9％与40．5％，P=0．004）。MMP-9的表达与Dukes分期、浸润深度、淋巴结转移正相关（rs=0．429，P=0．005；rs=0．372，P=0．015；rs=0．372，P=0．015）；TIMP-1的表达与Dukes分期、浸润深度、淋巴结转移正相关（rs=0．394，P=0．010；rs=0．382，P=0．013；rs=0．382，P=0．013）。MMP-9的表达与TIMP-1的表达呈正相关（rs=0．641，P〈0．001）。结论MMP-9和TIMP-1在大肠癌发生发展中起重要作用，联合检测二者对大肠癌侵袭转移的预测、预后评估及指导合理的综合治疗等具有重要的临床意义。
Objective To investigate the expression and clinicopathological significance of matrix metalloproteinase（MMP-9） and matrix metalloproteinase tissue inhibiting factor-1 （TIMP-1） in eolorectal carcinomas. Methods Immunohistoehemistry （PowerVision method） in the paraffin embedded tissue samples was used to determine MMP-9 and TIMP-1 in 42 eolorectal carcinomas and its adjacent normal colorectal mueosa. The relationship of their expression with some clinicopathological characteristics was analyzed. Results MMP-9 and TIMP-1 were both significantly overexpressed in colorectal carcinomas compared with its adjacent normal colorectal mucosa （69.1% and 61.9% vs. 45.2% and 40.5% ,P = 0.009 and P = 0. 004）, and MMP-9 was positively associated with the regional lymph node metastasis, bowel wall invasion and Dukes stage （ rs = 0. 372, P = 0.015 ; L = 0. 372, P = 0. 015 ; rs = 0.429 ,P = 0.005 ）. TIMP-1 was positively associated with Dukes stage, bowel wall invasion and regional lymph node metastasis （rs =0.394 ,P =0.010;r, =0.382,P =0.013 ;rs = 0. 382,P =0.013 ）. There was a positive correlation between the expression of MMP-9 and TIMP-1 （ rs = 0. 641, P 〈 0. 001 ）. Conclusion MMP-9 and TIMP-1 may play a key role in eoloreetal carcinogenesis. Examination of MMP-9 and TIMP-1 expression may have an important significance for evaluating prognosis, predicting of invasion and metastasis and comprehensive therapy.
出处
《中国综合临床》
2009年第2期143-145,共3页
Clinical Medicine of China
基金
唐山市科学技术研究与发展指导计划课题（06134601c-8）
