晚香玉组培技术研究

Establishment of Regeneration System from Leaves of Tuberose

以单瓣、重瓣两个晚香玉品种叶片为材料,选用4种升汞消毒时间、6种愈伤组织诱导培养基、6种分化培养基和9种生根培养基,研究不同基因型和激素组合对再生体系建立的影响。结果表明:(1)叶片的消毒时间为0.1%HgCl2浸泡2min;(2)诱导单瓣叶片愈伤形成的较适培养基为:MS+6-BA2.0mg/L+NAA1.0mg/L;诱导重瓣叶片愈伤形成的较适培养基为:MS+6-BA1.5mg/L+NAA0.5mg/L.(2)单瓣叶片分化最佳配方为:MS+6-BA1.0mg/L+NAA0.5mg/L;重瓣叶片分化的较适培养基为:MS+6-BA0.5mg/L+NAA0.2mg/L,(3)诱导生根的最佳培养基为:1/2MS+KT0.2mg/L+NAA0.5mg/L+6-BA0.2mg/L。

This paper deals with regeneration system from leaves of 2 Tuberose varieties （single petal tuberose and double petal tuberose ） with four different times of leaves sterilization with 0.1%HgCl2,six induced callus medium, six differentiation medium and nine rooting medium. The effects of genotype, hormone concentration and explants condition were investigated. The results showed that （1）The leaves sterilization with 0.1%HgCl2 for two minutes was the best;（2）The best culture medium for single petal callus inducement was MS＋6-BA2.0mg/L＋NAA1.0mg/L, and the best culture medium for double petal callus inducement was MS＋6-BA1.5mg/L＋NAA0.5mg/L;（3）The best culture medium for single petal shoot regeneration was MS＋6-BA1.0mg/L＋NAA0.5mg/L,and the best culture medium for double petal shoot regeneration was MS＋6-BA0.5mg/L＋NAA0.2mg/L;（4）The best medium for rooting was 1/2MS＋＋ KT0.2mg/L＋NAA0.5mg/L＋6-BA0.2mg/L.