RP-HPLC法分析测定燕麦中的酚类化合物

Analysis and Determination of Phenolic Compounds extracted from Oats through RP-HPL Method

利用高效液相色谱技术分析并鉴定了5种吉林产裸燕麦中的4种酚类化合物。高效液相色谱实验采用反相C18色谱柱,二级管阵列检测器,流动相采用乙腈和2%醋酸水溶液二元线性梯度洗脱,检测波长为280nm,通过与已知标准化合物的保留时间和紫外光谱相比较,鉴定了燕麦中的对羟基苯甲酸、丁香酸、对羟基苯甲醛和p-香豆酸4种化合物。实验中考察了不同时间碱水解条件下燕麦提取物中四种酚类化合物的含量,结果表明:4h碱水解提取效率较高。

RP - HPLC method was applied to analyze and determine four phenolic compounds extracted from five sorts of oats in Ji- lin： A reversed-phase C18 column and diode-array detector were applied in RP-HPLC experiment, moreover, the mobile phase was binary linear gradient eluted by acetonitrile and 2% acetic acid and the detective wave length was set as 280 nm. Then the 4 phenolic compounds： p-hydroxybenzoic acid, syringic acid, p-hydeoxybenzaldehyd and p-coumaric acid extracted from oats were identified based on comparing their retention times and UV spectrums obtained through HPLC experiments with those of the authentic standards. The contents of four phenolic compounds extracted from oats under different alkaline hydrolysis time were observed in this experiment. The results indicated that the extraction efficiency of 4-hour alkaline hydrolysis was higher.