邪毒壅盛证H_(22)荷瘤小鼠肿瘤组织基因的表达特征研究

Special Genes Expression in Tumor Tissues of H22 Mice with Obstruction of Evil Syndromes

目的揭示H22荷瘤小鼠邪毒壅盛证肿瘤组织基因表达的特征。方法采用GeneChip Mouse Exon 1.0 STArray等技术及芯片分析方法,观察H22荷瘤小鼠邪毒壅盛、早期气虚、中期阳气虚及中晚期气阴阳虚证肿瘤组织基因表达的差异。结果筛选到邪毒壅盛证独特性高表达的基因32个,其中包括Tcte3、Phf7、Ldhc等参与睾丸发育、精子或卵子发生的基因22个,Adipoq、Nrcam、Tnfaip6等与肿瘤有关的基因4个,以及Atp8b3、Pnpla3、Retn等其他功能的基因6个。筛选到邪毒壅盛证独特性低表达的基因2个,有Cort和LOC668417。结论邪毒壅盛证H22荷瘤小鼠肿瘤组织中存在大量独特性表达的基因,可能是该证候与其他证候区别的内在特征。

Objective To investigate genes expression profile in tumor tissues of H22 mice with obstruction of evil syndromes. Methods Genes expression in the tumor tissues of H22 mice with syndromes of obstruction of evil syndromes, ＂Qi＂ deficiency, deficiency of ＂Qi and Yang＂, or deficiency of ＂Qi Yin and Yang＂, were analyzed by Affymetrix GeneChip Mouse Exon 1.0 ST Array. Results 32 special higher - expression genes were observed, including 22 genes which involve in testicle growth, sper- matogenesis or oogenesis, 4 genes related to tumors, and 6 other gene. Simultaneously, 2 special lower - expression genes were observed. Conclusion Numerous genes have special expression in the tumor tissue of H22 mice with obstruction of evil syndromes, which may be intrinsic difference between this syndrome and 3 other syndromes.