生姜油不同部位对肝损伤模型大鼠肝细胞保护作用的比较

Comparison of the Protective Effects of Different Parts of Ginger Oil against Rat Hepatocytes in vitro

目的比较生姜油3个不同部位对四氯化碳(CC l4)损伤培养的大鼠肝细胞的保护作用及可能作用机制。方法用大鼠制备含药血清。利用CC l4体外诱导大鼠肝细胞损伤模型,用比色法检测肝细胞经含药血清处理后上清液中谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)和超氧化物歧化酶(SOD)水平的变化。结果生姜油A部位含药血清能显著降低ALT和AST水平,提高SOD活力,抑制MDA的产生;B部位亦能显著降低ALT和AST水平,提高SOD活力,但对MDA未见有明显的影响;C部位仅能降低AST水平,提高SOD活力。结论生姜油3个不同部位的含药血清对CC l4引起的大鼠肝细胞损伤均有不同程度的保护作用,但以A部位的效果较好,其保护机制与其抗氧化作用有关。

Objective To compare the protective effects of three parts of ginger oil against rat hepatocyte injury induced by carbon tetrachloride （ CCl4 ） and its mechanisms in vitro. Methods The serum - drug of three parts of ginger oil was prepared with SD rats. The model of hepatocyte injury induced by CCI4 was used to examine the effects of serum - drug on the levels of alanine ami- notransferase （ ALT）, aspartate aminotransferase （ AST）, malondialdehyde （MDA） and superoxide dismutase （SOD） in cultured supernatant. Results Part A of ginger oil could significantly decrease the levels of ALT, AST and MDA, increase the activity of SOD. Part B also significantly decreased the levels of ALT and AST, increased the activity of SOD. However it didnt inhibit the production of MDA. Part C only decreased the level of AST, increased the activity of SOD. Conclusion The three parts of ginger oil can prevent CCl4 - induced hepatocyte injury in some degree, but the protective effect of part A is the best, and it is related to its antioxidation.