摘要
目的观察加味四逆散对肝纤维化大鼠肝组织Ⅰ,Ⅲ,Ⅳ型胶原的影响效果,从而探讨该方抗肝纤维化的机理。方法采用二甲基亚硝胺腹腔注射制备大鼠肝纤维化模型,加味四逆散灌胃给药,免疫组织化学方法检测分析各组大鼠肝组织Ⅰ,Ⅲ,Ⅳ型胶原的含量。结果加味四逆散能明显降低肝纤维化大鼠肝组织Ⅰ,Ⅲ,Ⅳ型胶原的含量,与病理模型组比较有显著性差异(P<0.05)。结论加味四逆散可有效的减少肝组织Ⅰ,Ⅲ,Ⅳ型胶原的沉积,对肝纤维化有肯定的治疗作用。
Objective The mechanism of Chinese herbail compound JIA WEI SI NI SAN in treating hepatic fibrosis were explored and studied by observing the expression of Collagen Ⅰ,Ⅲ and Ⅳ Collagen in liver. Methods The rat fibrosis model was induced by DMN. The groups of treatment were fed with JWSNS garage. Ⅰ,Ⅲ and Ⅳ collagen were detected in liver samples with immunohistochemisty. Results Upon pathological examination , the JWSNS treatment had significantly reversed DMN - induced liver f brosis . In JWSNS treatment group the expression of collagen Ⅰ,Ⅲ and Ⅳcollagen in liver was markedly reduced respectively according to immunohistochemisty analysis as compared with model group（ P 〈 0.05 ）. Conclusion The mechanism of treating hepatic fibrosis with JWSNS appears to due to the enhancement of degradiation of collagen in liver. JWSNS can treat hepatic fibrosis effectively.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第1期177-178,共2页
Lishizhen Medicine and Materia Medica Research
