利用STS标记检测CIMMYT小麦品种(系)中Lr34／Yr18、Rht-B1b和Rht-D1b基因的分布

Characterization of Lr34/Yr18,Rht-B1b,Rht-D1b Genes in CIMMYT Wheat Cultivars and Advanced Lines Using STS Markers

【目的】明确慢锈基因Lr34/Yr18和矮秆基因Rht-B1b(Rht-1)、Rht-D1b(Rht-2)在CIMMYT小麦品种中的分布,帮助慢病性品种选育和株高改良。【方法】使用3个STS标记,检测慢锈基因Lr34/Yr18和矮秆基因Rht-B1b、Rht-D1b在263个CIMMYT小麦品种和高代品系中的分布。【结果】csLV34标记在含Lr34/Yr18的材料中扩增出一条150bp的特异带,在不含Lr34/Yr18的材料中扩增出229bp的特异带;利用2对互补引物NH-BF.2/WR1.2和NH-BF.2/MR1对Rht-B1a和Rht-B1b基因进行检测,在携带Rht-B1a和Rht-B1b的材料中分别扩增出一条400bp的特异带;利用DF/MR2标记检测Rht-D1b基因,在携带Rht-D1b的材料中,扩增出一条280bp的片段。在263个品种中,57个品种携带Lr34/Yr18基因,占总数的21.7%;216个品种含Rht-B1b,占总数的82.1%;38个品种含Rht-D1b,占总数的14.4%。含双矮秆基因型(Rht-B1b+Rht-D1b)的品种有12个,不含这2个矮秆基因的品种(Rht-B1a+Rht-D1a)有21个。【结论】这3个STS标记可以方便、快速、准确地检测Lr34/Yr18、Rht-B1b和Rht-D1b基因。在CIMMYT材料中,Rht-B1b基因频率很高,Rht-D1b较低。

[Objective] Characterization of genes Lr34/Yr18, Rht-Blb （Rhtl） and Rht-D1b （Rht2） in 263 CIMMYT wheat cultivars and advanced lines will benefit the improvement of rust resistance and plant height in Chinese wheat breeding program. [ Method ] A total of 263 CIMMYT wheat cultivars and advanced lines were tested by STS markers to understand the distribution of resistance genes Lr34/Yr18 and dwarfing genes Rht-Blb and Rht-D1b. [Result] The marker csLV34 could amplify a 150 bp fragment in the lines with Lr34/Yr18, and a 229 bp fragment in those lines without Lr34/Yr18. Two complementary markers, NH-BE2/WR1.2 and NH-BE2/MR1, were used to investigate the alleles Rht-B1a and Rht-B1b, respectively. They could amplify a 400 bp fragment in the genotypes with Rht-B1a and Rht-B1b, respectively. The marker DF/MR2 could generate a 280 bp fragment in the genotype with Rht-Dlb. Of the 263 lines, 57 generated a 150 bp fragment with the marker csLV34, indicating the presence of Lr34/Yr18 in these lines, with a frequency of 21.7%. Two hundred and sixteen lines （82.1%） were detected to have the allele Rht-B1b, and 38 lines （14.4%） contained the allele Rht-Dlb. Twenty-one lines were detected to possess both the wild-type alleles Rht-Bla and Rht-Dla, while 12 had two dwarfing genes Rht-B1b and Rht-D1b. [Conclusion] These STS markers could be useful to detect the genes Lr34/Yr18, Rht-Blb and Rht-D1b. The frequency of Rht-Blb was much higher than that of Rht-D1b in CIMMYT lines.