猪POU1F1第一内含子单核苷酸多态性和生长性状相关性研究

Relationship of Single Nucleotide Polymorphism of Intron 1 in Procine POU1F1 and the Growth Trait

【目的】寻找猪POU1F1多态位点和基因表达量之间可能存在的相关性。【方法】以大白猪(英系)和中畜黑猪为试验材料,对POU1F1基因第一内含子多态性进行PCR-SSCP检测分析,同时提取垂体总RNA,对POU1F1、GH、PRL、TSH-β的mRNA进行实时定量荧光PCR检测。【结果】发现一个位于第一内含子1515位点的突变和猪屠宰前日增重相关系数为R=-0.6(P<0.05),且该位点的替换和屠宰前GH的表达量存在显著相关(R=0.483,P<0.05);【结论】T→C的替换将导致GH的mRNA表达量升高,在生长性状上表现为屠宰前日增重降低。

[Objective] The relationship between porcine DNA polymorphisms of POU1F1 and mRNA expression was studied. [ Method ] The intron 1, a partial regional polymorphisms of porcine POU1F1 of two breeds of Yorkshare and Zhongxu black, were investigated by PCR-SSCP. Based on the total RNA contracted from animals pituitary, the mRNA quantities of POU1F1, GH, PRL, and TSH-β were masured by RQ-PCR. [Result] It was found that a SNP of T→C in intron 1 （site 1515bp）, markedly correlated with the daily weight gain （R=-0.6, P〈0.05） and the expression of GH （P〈0.05） before slaughtering. [Conclusion] The SNP of T→C led to the increase of the GH expression; But the dally weight gain decreasd in the growth trait before slaughtering.