CdSe量子点作探针共振瑞利散射法测定阿米卡星

Resonance rayleigh scattering spectral method for determination of AMK with CdSe QDs as probe

以巯基乙酸为稳定剂在水相中合成CdSe量子点,巯基乙酸自组装在量子点的表面形成带有负电的聚集体.在pH为4.6～6.3的中性或弱酸性介质中,可与阿米卡星(Amikacin AMK)靠静电引力及疏水作用力结合形成粒径较大的聚集体,这种聚集体的形成导致共振瑞利散射(Resonance Rayleigh Scattering RRS)强度显著增大,最大散射峰位于372nm.同时二级散射(second-order scattering SOS)和倍频散射(frequency-doubling scattering FDS)的强度也显著增强,最大峰分别位于590nm、392nm.其中,RRS方法有较高的灵敏度和较低的检出险,它的线性范围为0.0086～1.2μg·mL-1,检出限为2.66ng·mL-1.以此建立一种用CdSe量子点作为探针RRS法测定AMK的高灵敏,简便,快速新方法,同时讨论了最佳反应条件和其它影响因素,该方法可用于血样中AMK的测定.

Thioglycolic acid （TGA） is employed as stabilizing agents to synthesize CdSe quantum dots. In the neutral or low-acid medium（ pH is between 4.6 - 6.3 ）, Amikacin （AMK） with univalent positive charges can be combined with the CdSe-QDs by the virtue of electrostatic and hydrophobic interaction, forming bigger diamete aggregate. The aggregate arouses Resonance Rayleigh Scattering （ RRS ） intensity enhancing greatly, the maximum wavelengths located at 372 nm. At the same time, the second-order scattering （ SOS ） and frequency-doubling scattering （ FDS ） intensities are also enhanced, the maximum wavelengths located at 590 nm and 392 nm respectively . Among them, the RRS method has a higher sensitivity and lower detection limit, its linear range is 0.0086 -1.2 μg ·mL^-1 and detection limit is 2.66 ng· mL-1. A simple, fast and sensitive method that CdSe-QDs uses as the RRS probe for the determination of AMK is developed. Also the optimum reaction conditions and influencing factors are discussed. It has been applied to the determination of AMK in human serum with satisfactory results.