土鳖虫质量优化前后主要成分的分析

Analysis the main component of Eupolyphaga sue Steleophaga before and after quality optimization

目的对质量优化前后的土鳖虫药材进行质量比较研究,分析质量优化前后土鳖虫的成分差异。方法按2005版《中国药典》一部附录ⅨH水分测定法第一法、附录ⅨK灰分测定法、附录ⅩA浸出物测定法对土鳖虫药材的水分、总灰分、酸不溶灰分、水溶性浸出物等质量方面进行了研究,采用凯氏定氮法进行粗蛋白的测定,采用电感耦合等离子发射光谱进行微量金属的测定。结果质量优化前后的土鳖虫药材的总灰分、酸不溶性灰分、水分含量没有很大差异,符合《中国药典》中质量检查项规定。质量优化前后土鳖虫药材中粗蛋白含量和微量元素含量有明显差异。结论质量优化后的土鳖虫明显优于质量优化前,探索的优化养殖方法,为建立土鳖虫药材的生产质量管理规范提供了可供参考的数据资料。

OBJECTIVE To compare the quality of Eupolyphaga sue Steleophaga before and after quality optimization, and analysis the compenent differences. METHODS Detemination of the contents of moisture,total ash,acid-insoluble ash,water-soluble extract and crude protein according to the appendix methods of Chinese Pharmacopoeia 2005. Trace elements were determined by inductively coupled plasma-atomic emission spectrometry （ICP-AES）. RESULTS There is no difference in the terms of the contents of moisture,total ash,acid-insoluble ash,water-soluble extract between the Eupolyphaga sue Steleophaga before and after quality optimization. But the contents of crude protein and trace elements are different. CONCLUSION After quality optimization,the quality of Eupolyphaga sue Steleophaga is better. The optimizated culture method could provide refering datas for Eupolyphaga sue Steleophaga GAP Cultivation.