氟伐他汀对产科抗磷脂综合征患者血清活化内皮细胞的影响

Fluvastatin inhibits endothelial cell activation induced by the serum of patients with obstetric antiphospholipid syndrome

目的:研究氟伐他汀对产科抗磷脂综合征(APS)患者血清活化内皮细胞的影响。方法:分别用APS患者血清、健康对照血清及培养基处理人脐静脉内皮细胞(HU-VEC),其中APS血清处理组预先加入不同浓度的氟伐他汀(0、2.5、5和10μmol/L)干预,用逆转录定量聚合酶链反应(RT-PCR)检测脐静脉内皮细胞上细胞间黏附分子-1(ICAM-1)mRNA表达,用流式细胞仪检测其蛋白的表达。结果:与对照组相比,APS患者血清可诱导ICAM-1 mRNA和蛋白表达增加,而氟伐他汀干预对ICAM-1 mRNA和蛋白表达有不同程度的抑制,且呈浓度依赖性(P<0.05)。结论:氟伐他汀可减少脐静脉内皮细胞黏附分子表达,影响抗磷脂综合征患者血管内皮活化。提示他汀类药物可为治疗APS提供新的选择。

Objective：To investigate whether fluvastatin interferes with endothelial cell （EC） activation induced by the serum of patients with obstetric antiphospholipid syndrome. Methods：Human umbilical vein endothelial cells were treated with serum from APS patients, normal controls, medium respectively. Cells were pretreated with fluvastatin （0,2.5,5 and 10μmol/L） before treated with APS serum. ICAM-1 mRNA was measured by reverse transcriptase-polymerase chain reaction and its antigen by flow cytometry. Results： Incubation of EC with APS serum led to a higher ICAM-1 expression compared with incubation with control serum. Fluvastatin treatment of the cells significantly reduced the ICAM-1 response to APS serum, and this effect was dose dependent （P 〈 0.05 ）. Conclusions： Fluvastatin exerts a protective effect towards APS serum at the EC level by decreasing leukocyte adhesion molecule expression on endothelial cells. This data provide a rationale for using statins as a therapeutic tool in treatment of APS.