子痫前期患者胎盘组织氧化应激与PeroxiredoxinⅡ

Oxidative stress in placenta of pre-eclampsia and PeroxiredoxinⅡ

目的:研究子痫前期胎盘组织氧化应激情况及PeroxiredoxinⅡ的表达,探讨PeroxiredoxinⅡ在子痫前期氧化应激机制中的作用。方法:用蛋白免疫印迹法检测15例子痫前期患者及15例正常妊娠者胎盘组织中PeroxiredoxinⅡ表达,测定相应组织中SOD活力及MDA、H2O2含量。结果:子痫前期患者胎盘组织中,PeroxiredoxinⅡ的表达明显低于正常妊娠组(P<0.05)。子痫前期组氧化应激指标SOD活力为89.74±18.26U/mg-prot,明显低于正常妊娠组105.23±21.88U/mgprot(P<0.01);MDA含量96.69±32.88nmol/mgprot,明显高于对照组63.29±13.74nmol/mgprot(P<0.05);H2O2含量为81.65±17.93nmol/mgprot,显著高于对照组的68.58±13.55nmol/mgprot(P<0.01)。H2O2含量与PeroxiredoxinⅡ表达量呈负相关。结论:PeroxiredoxinⅡ与子痫前期发生发展中的氧化应激机制存在密切关系。

Objective： To investigate the role of peroxiredoxin Ⅱ （ Prx Ⅱ ） and oxidative stress in placenta of pre-eclampsia. Methods： The expression of Prx Ⅱ in placenta was determined by Western blot and compared between 15 cases pre-eclampsia pregnancies and 15 cases normal pregnancies. The homogenates of placenta were estracted and the aeitivity of superoxide dismutase （SOD） and the quantity of malondialdehyde （MDA） , hydrogen peroxide （ H2O2 ） were measured. Results：The expression of Prx Ⅱ in pre-eclampsia was significantly lower than that in normal pregnancies （ P 〈 0.05 ）. The activity of SOD of homogenate of placenta in preeclampsia was 89.74 ± 18.26U/mgprot, lower than that of control 105.23 ± 21.88U/mgprot （ P 〈 0.05 ） ,while MDA was 96.69 ± 32.88nmol/mgprot, higher than that of control 63.29 ± 13.74nmol/mgprot. So as H2O2 in pre-eclampsia with the quantity 81.65 ± 17.93nmol/mgprot,which was higher than that of control 68.58 ±13.55nmol/mgprot （P 〈 0.05 ）. Negative correlation between H2O2 and Prx Ⅱ was found both in study and control group. Conclusion： Prx Ⅱ has a close relation with the develot）ment of pre-eclampsia.