早期慢性移植肾肾病大鼠的肾组织学改变及转化生长因子-β1的表达

The renal histological change and the renal expression of TGF-β1 in the early stage of chronic allograft nephropathy

目的探讨早期慢性移植肾肾病（CAN）大鼠的组织学改变与转化生长因子-β1（TGF-β1）表达的关系。方法以F344大鼠为供体，Lewis大鼠为受体，建立大鼠CAN模型，分为移植组、F344对照组和Lewis对照组。观察3组大鼠肾功能、肾组织学改变及。肾组织TGF-β1的表达。结果①与F344对照组和Lewis对照组比较，移植组大鼠24h尿蛋白及血肌酐明显升高，差异有统计学意义（P〈0．05），肾组织改变以单个核细胞浸润为主，肾组织TGF-t31表达水平升高，差异有统计学意义（P〈0．05）；②移植组大鼠肾组织单个核细胞浸润与TGF-β1表达呈正相关，慢性病变与TGF-β1表达无相关性。结论早期CAN功能学上表现为出现蛋白尿、血肌酐升高，组织学上表现为以单个核细胞浸润为主，并与TGF-β1表达升高呈正相关。

Objective To observe the renal histological change and the renal expression of TGF β1 in the early stage of chronic allograft nephropathy. Methods The rats were divided into three groups（n = 5） ：F344-Lewis group.F344 group and Lewis group. The renal histological change and the renal expression of TGF-β1 were examined after 12 weeks. Results ①Compared with the rats in F344 group and Lewis group, the level of urine protein, serum ereatinine, mononuclear cell infiltration. TGF- β1 expression in F344-Lewis group rats were increased（P〈0. 05）; ②Mononuelear cell infiltrations were positive correlationed with the renal expression of TGF-β1 （P〈0. 05）, Chronic pathologic changes had no significant correlation with TGF-β1 expression. Conclusions In the early stage of CAN, the level of urine protein, serum creatinine were increased, Mononuclear cell infiltrations were positive correlationed with the renal expression of TGF-β1.