摘要
对光活化金丝桃素体外抑制口蹄疫病毒与宿主细胞吸附作用进行实验。体外培养的BHK-21细胞加入金丝桃素,日光灯下光活化1 h后感染FMDV。通过细胞病变观察、MTT法、双抗体捕获抗原法、扫描电子显微镜进行抑制FMDV与宿主细胞吸附、融合研究。结果表明金丝桃素有明显抑制细胞病变的作用。双抗体捕获抗原法检测金丝桃素有效抑制FMDV和BHK-21细胞的吸附、融合,抑制率可达59.72%。扫描电镜观察,金丝桃素组与病毒对照组相比,BHK-21细胞FMDV颗粒明显减少。提示金丝桃素在体外有显著抑制口蹄疫病毒与宿主细胞的吸附、融合作用。
Objective:to determine the inhibitory effects of phoyodynamic hypericin on the adsorptiveability of Foot-and-Mouth virus(FMDV)tohost cells in uitro.Mehods:BHK-21 cells infected by FMDV were treated with different dose of hypericin byinduced-visble-light,then cytopathic effect observation,MTT assay,double-antibaby trapped ELISA and seanning electron microscope were used to derect the adsorption and fusion of FMDV to host cells.Results:Hypricin could significantly inbibit cytopath-ic effectof BHK-21 cell induced by FMDV.Itcould also strongly inhibit the adsorptive ability of FMDV to BHK-21 cell with maxi-mal inhibitory rate of 59.72%.Scanning electron microscope showed that there were lcsser virus particle of FMDV in BHK-21 cell after using hypericin.Conclusion:Hypericin could effectively inhibit the adsorption and fusion of FMDV to host cells in vitro.
出处
《中兽医医药杂志》
2009年第1期5-8,共4页
Journal of Traditional Chinese Veterinary Medicine
基金
十五国家攻关项目(2002BA514A-17-07)