四环素抗性基因PCR检测及酶切分析的实验与临床研究

Laboratory and clinical study on PCR detection and restrictive endonuclease analysis to tetracycline resistant determinant (tet M)

采用PCR技术对551份泌尿生殖道分泌物标本进行四环素抗性基因（tetM）检测，并进行敏感性及特异性试验、应用Taq-1限制性内切酶对PCR产物进行酶解消化反应结果表明四环素抗性基因决定子的阳性检出率为36．84％（20／551），大多数PCR产物在酶解后可产生3个预期酶解片段（190bp、130hp及77hp），少数则缺如此3个酶解片段。研究结果提示，用PCR方法可敏感、快速、准确及大批量筛查tetM耐药基因，在tetM决定子内可能存在核苷酸序列上的差异。

PCR technique was used to detect tet Mdetrminant in 551 specimens of urogenital discharges and restrictive endonuclease analysis was used to confirm the PCR result and discover the difference among drug resistant strains. The results showed that the posititve rate of tet M was 36. 849 (203/551 ). Most PCR products produced 3 expected fragments (190. 130 and 77bp ) after digested with Taq- 1 endonuclease. The results showed that tet M could be detected by PCR rapidly. accurately and on large scale. and some different endonuclease digested patterns of tet M determinant may be existed in some strains.