鼻分泌物及皮肤组织中麻风菌及其PGL-1抗原的检测

Detection of M.leprae and PGL-1 antigen in nasal secretion and skin tissue

为了更好地理解麻风菌鼻携带在麻风病传播、维持中的作用，以及运用鼻携带检测来评价麻风病防治效果，比较了PCR和Dot-ELISA／ECL平行检测32例活动性麻风患者、13例愈后者和143名麻风家内接触者鼻分泌物及皮肤组织中的麻风菌及其PGL-1抗原。结果显示，Dot-ELISA／ECL具有较好的敏感性、特异性，是一项适用于现场研究的简便、快速、经济的麻风流行病学工具。此外，用于免疫学试验，GVHP是一种吸附性好，适合于检测粘膜分泌物抗原的载体。

In order to better understand the role of nasal carriage of M. leprae in the transmission of leprosy and evaluate the efficacy in leprosy control with detection of M. leprae in nasal secretion. PCR and Dot-ELISA/ECL were applied to detect M. leprae and PGL-1 antigen in nasal secretion and skin tissue samples from 32 active leprosy patients, 13 cured patients and 143 household contacts. The comparititive study showed that Dot-ELISA/ECL is a sensitive, specific, simple and rapid method, it can be used in the epidemiologic study on leprosy. In addition. GVHP membrane is a good absorbent earner siutable for the detection of mucosa-secreted antigen.