多巴胺和谷胱甘肽对PC_(12)细胞凋亡的影响

Effect of dopamine and glutathione on the apoptosis in PC12 cell

目的研究帕金森病（ＰＤ）黑质多巴胺神经元的死亡机制。方法用脱氧核糖核酸（ＤＮＡ）标记法，借助荧光显微镜观察多巴胺（ＤＡ）和还原型谷胱甘肽（ＧＳＨ）对ＰＣ１２细胞凋亡的影响。结果发现ＤＡ可诱发ＰＣ１２细胞凋亡，适中浓度时（０．４５ｍｍｏｌ／Ｌ）ＰＣ１２细胞凋亡数最多（凋亡率为４８．７％±６．３％）；抗氧化剂还原型谷胱甘肽（ＧＳＨ）可部分阻止ＤＡ诱发的ＰＣ１２细胞凋亡（Ｐ＜０．０１）。结论凋亡参与了ＰＤ的病变过程，采用适当的抗氧化剂对于ＰＤ治疗具有积极的意义。

Objective To study the mechanism of dopaminergic neuronal death in substantia nigra of Parkinson,s disease(PD).Method The effect of dopamine(DA) and glutathione (GSH) on apoptotic PC12  cell were examined by fluorescence microscopy using the DNA endlabeling methods.Results It has been demonstrated that DA may induce apoptosis in PC12 cell,moderate concentrations of DA (0.45mmol/L) gave maximal number of apoptotic cell (the rate of apoptosis:48.7%±6.3%);and the antioxidant reduced glutathione(GSH) may partially prevent DAinduced apoptosis in PC12 cells (P<0.01).Conclusion Apoptosis may be involved in the pathogenesis of PD,and treatment with proper antioxidants may have positive influence on the therapeutic strategy for PD.