人脑胶质瘤WAF_1/CIP_1表达的研究

Studies on the expression of WAF 1/CIP 1 in human glioma

目的检测抑癌基因ＷＡＦ１／ＣＩＰ１在人脑胶质瘤中的表达。方法取４６例胶质瘤组织及１２例脑外伤组织，提取其ｍＲＮＡ，用ＷＡＦ１／ＣＩＰ１作为模板进行逆转录ＰＣＲ（ＲＴ－ＰＣＲ）。结果４６例胶质瘤中有１４例有ＷＡＦ１／ＣＩＰ１蛋白表达（１４／４６），表达率为３０．４％，１２例脑外伤标本全部表达（１２／１２），表达率为１００％，两组比较有显著差异（Ｐ＜０．０１）。结论ＷＡＦ１／ＣＩＰ１的缺失是脑胶质瘤发生及进展的原因之一。

Objective To study expression of cancersuppressing genes WAF 1/CIP 1 in human glioma.Method To take up the mRNA from 46 cases with brain glioma and 12 cases with brain contusion,the WAF 1/CIP 1 RNA are carry out re frans formation PCR.CRI PCR.Results 14 out of 46 cases with brain glioma show WAF 1/CIP 1(30.4%),while all in 12 cases with contusion show WAF 1/CIP 1(100%),there is a significant differece between the two groups (P<0.01).Conclusion Defect WAF 1/CIP 1 is an important cause in developing human glioma.