摘要
目的：为了进一步了解斑点免疫结合试验（ＤＩＢＡ）诊断包虫病的敏感性和特异性，进行血清特异抗体的检测，并与间接血凝试验（ＩＨＡ）和对流免疫电泳试验（ＣＩＥＰ）作比较。方法：用羊肝细粒棘球蚴液抗原对９４例包虫病患者、２２例非包虫病患者和４５例健康人血清进行了ＤＩＢＡ、ＩＨＡ和ＣＩＥＰ。结果：ＤＩＢＡ、ＩＨＡ、ＣＩＥＰ的阳性率分别为９２．６％、８６．２％和６３．８％，ＤＩＢＡ的阳性率明显高于ＣＩＥＰ（Ｐ＜０．０１），而与ＩＨＡ无统计学差别；假阳性率分别为５．８％、９．０％和０。结论：ＤＩＢＡ的敏感性高于ＩＨＡ和ＣＩＥＰ，且与ＣＩＥＰ有显著差异；ＤＩＢＡ的特异性高于ＩＨＡ，但低于ＣＩＥＰ。在诊断包虫病时，应同时使用两种以上免疫试验。
Objective: To further understand the sensitivity and specificity of DIBA in diagnotic value for human hydatidosis, and to contrast the IHA with CIEP. Methods: DIBA, IHA and CIEP were performed with antigen from sheep's hepatic hydatid fluid on sera of 94 patients with hydatid disease, 22 patients of non hydatid disease and 45 healthy adults. Results: The positive rates of the DIBA, IHA and CIEP were 92.6%,86.2% and 63.8% respectively, DIBA was much higher than the CIEP (P<0.01), but no significant difference between the DIBA and IHA. The pseudo positive rates of DIBA, IHA and CIEP were 5.8%, 9.0% and 0 respectively. Conclusions: The sensitivity of the DIBA is higher than the IHA and CIEP, the difference is significant between DIBA and CIEP; Specificity of the DIBA is higher than the IHA, but lower than the CIEP. More than two kinds of immunity test should be used in the same time on the diagnosis of hydatid disease in order to increase the accuracy rate of diagnosis.
