摘要
目的：进一步评价多聚酶链式反应（ＰＣＲ）技术在诊断结核性胸膜炎中的使用价值。方法：采用涂片法和ＰＣＲ技术，对７４例（结核性４２例，癌性３２例）胸腔积液进行检测，并加以对照和比较。结核病例组和肺癌对照组均按金标准产生，两组的一般情况具有可比性。结果：涂片法检查结核性胸液阳性率为９．５％，对照组阳性率为３．１％；ＰＣＲ检测结核性胸液阳性率为７１．４％，对照组阳性率仍为３．１％。两法相比，ＰＣＲ技术灵敏度明显高于涂片法。结论：提示ＰＣＲ技术在结核性胸膜炎的病原学诊断中具有较高的优越性。
Objective: In order to further evaluate the value of PCR technique in the diagnosis of tuberculous pleurisy. Methods: The pleural fluids of 42 tuberculous patients were studied with PCR technique and in comparison with smear examination simultaneusly and the pleural fluids of 32 patients of lung cancer were also studied in the same manner as control group. The tuberculous group and the control group were defined by the Gold Standard, and their general characteristics of two groups were comparable. Results: The results by smear examination showed that the positive rate in tuberculous group was 9.5% and in control group was 3.1%, while by PCR technique, both were 71.4% and 3.1% respectively. Conclusions: The PCR technique may possess of superiority in the diagnosis of tuberculous pleurisy.
