川芎嗪预处理对大鼠缺血再灌注肝脏的保护作用

The effect of N-acetylcysteine on expression of nuclear factor-κB on hepatic ischemia infury in rat models

目的探讨川芎嗪(TMP)对大鼠缺血再灌注(I/R)诱导的急性肝损伤(AHI)大鼠的保护作用及对核因子(NF)-κB表达的影响。方法30只大鼠随机均分成假手术组(a组);I/R(b组);TMP干预组(c组)。b、c组均阻断肝大部血流后恢复灌注,其中c组于阻断血流前1h,腹腔注射TMP(30mg/kg)。各组大鼠再灌注3h后开腹,采血,切取肝左中叶常规病理检测:免疫组织化学染色(ABC法)检测NF-κB在肝组织中的表达。结果b组大鼠血清丙氨酸转氨酶(ALT)与天冬氨酸转氨酶(AST)的活性、中性粒细胞(PMN)计数及核因子(NF)-κB表达比均明显高于a组(P<0.01),肝脏在光镜与电镜下的显微与超微结构损伤明显;c组的各项指标均明显好于b组(P<0.01),但差于a组(P<0.01)。结论川芎嗪对急性肝缺血再灌注损伤具有保护作用,其作用机制可能是通过抑制NF-κB表达、PMN激活而实现。

Objective To explore the role of tetramethylpyrazine on the expression of nuclear faetor-kB （NF-kB） of rats with hepatic ischemia-reperfusion （I/R） injury, and study the possible mechanism of hepatic protection affected by TMP against acute I/R injury. Methods Thirty male Wistar rats were divided into three groups in randomly and average. group a for sham operation; group b for ischemia-reperfusion group c injected with N-aeetyleysteine before I/R. 3 hours later the rats were killed and the serum was taken to detect the ALT, bilirubin, The expression of NF-KB in heptie tissues was detected by immunohistochemical method. Results The expression of NF-KB and the level of ALT, bilirubin, PMN increased markedly during heptic ischemia-reperfusion compared to sham group （P〈0.01）; decreased significantly after administration of tetramethylpyrazine compared to I/R group （P〈0.01）. Conclusion Tetramethylpyrazine can protect heptie from acute I/R injury, partly through inhibiting activation of NF-KB and PMN to alleviate inflammation.