肝脏胞外基质成分参与卵圆细胞介导的肝再生

Hepatic extracellular matrix components participate in oval cell-mediated liver regeneration

目的探讨肝再生中肝脏胞外基质成分与卵圆细胞的相互关系及作用。方法采用免疫组化及免疫荧光双标的方法动态观察大鼠卵圆细胞增殖模型中肝脏胞外基质成分（层粘连蛋白和纤维连接蛋白）的定位及与卵圆细胞的关系。结果肝部分切除后第2天,卵圆细胞开始向门静脉周围区域增殖。层粘连蛋白主要出现在门静脉周围的肝窦状隙内,纤维连接蛋白在整个肝小叶内表达显著增加。术后第4～9天,卵圆细胞进一步向肝实质内增殖,门静脉周围纤维连接蛋白表达增加,中央静脉周围表达减少。层粘连蛋白及纤维连接蛋白与卵圆细胞关系紧密。术后第12～15天,随着卵圆细胞分化为小肝细胞结节,大多数层粘连蛋白及纤维连接蛋白位于结节周边,少数出现在结节内。第18天以后,正常的肝小叶结构开始恢复。结论卵圆细胞与肝脏胞外基质成分存在紧密联系;局部肝脏微环境可能通过细胞与基质之间的相互作用在卵圆细胞介导的肝再生中发挥重要的调控作用。

Objective To explore the relationship and interaction between extracellular matrix （ECM） components and oval cells （ OC ） during the oval cells-mediated liver regeneration. Methods A dynamic study on the localization of and the relationship of OC with ECM components （ laminin and fibronectin ） during the proliferation and differentiation of OC in 2-AAF/PH rats model was conducted and analysed by using try and double immunofluoreseent method. Results By day 2 after partial hepatectomy, OC began to proliferate around the portal area. Most of laminin were present along the hepatic sinusoids in the periportal area. Fibronectin markedly increased in the whole hepatic lobule. From day 4 to 9, OC spread further into hepatic parenchyma, closely associated with fibronectin and laminin. There was a decrease in number of the pericentral fibronectin and increase of fibronectin in the periportal areas. From day 12 to 15, as the OC differentiated into small hepatocellular nodus, most of laminin and fibronectin were located around the hepatocellular nodus, and few of them were present into the nodus. After day 18, the normal liver lobule structures began to recover. Conclusions There is a close relationship between the ECM components and OC during the liver restitutive repair. Local hepatic microenvironment may participate in the oval cell-mediated. liver regeneration through the cell-matrix interactions.