肿瘤干细胞的分离与纯化研究进展

Isolation and purification of tumor stem cells

随着肿瘤干细胞学说的日趋成熟,针对干细胞的靶向治疗已成为新的理论,分离与纯化肿瘤干细胞是研究的重要组成部分。目前国内外主要根据肿瘤干细胞已知的细胞表型和生物学特性,用流式细胞仪分选法、磁性激活细胞分选法和旁群细胞分选法来分离与纯化肿瘤干细胞,为肿瘤的临床诊断与治疗提供了依据。

With the development of the tumor stem cell theory, targeted therapy on stem cells has become a new theory. How to separate and purify tumor stern cells has also become an important part of the research. At present, according to the known cell phenotype and bionomics, tumor stem cells are separated and purified by fluorescence activated cell sorting, magnetic-activated cell sorting and the side population cell sorting method. These have provided protection for tumor clinical diagnosis and treatment.